16
posted ago by bob2112 ago by bob2112 +17 / -1

This is one of the email to fauci,. Copy and pasted from other article So this one is not real! Duck Duck go intervirion fusion and you find the same article Intervirion Fusion. HIV-luc(ACE2) (500 ng of p24) was mixed with 1,000 ng of p24 of HIV-gfp particles incorporating ASLV-A envelope, SARS-CoV S protein, or both envelopes in PBS at 4°C for 30 min to allow binding. Samples were raised to 37°C for 15 min to allow for conformational rearrangements. Virions were adjusted to the desired pH with 0.1 M citric acid. PBS, TPCK-trypsin (final concentration 10 gml), CTSL, cathepsin B (CTSB) (final concentrations 2 gml) or CTSL buffer alone was then added. Recombinant CTSL (R & D Systems) was preactivated by incubation for 15 min at 10 gml in 50 mM Mes, pH 6.0, on ice. Recombinant CTSB (R & D Systems) was preactivated in 25 mM Mes, 5 mM DTT, pH 5.0, for 30 min at 25°C. After a 10-min incubation at 25°C, proteolysis was halted by the addition of 300 l of DMEM10 containing leupeptin (25 gml) and STI (75 gml). Virions were then incubated at 37°C for 30 min to allow membrane fusion. 100 l of the virion mixture was added in quadruplicate to HeLa-Tva cells pretreated for 1 h with leupeptin (20 gml). The cells were spin-infected and incubated at 37°C for 5 h. The medium was replaced with fresh DMEM10 and the cells were assayed for luciferase activity 40 h later. Temperature-Sensitivity Intervirion-Fusion Assay. Intervirion-fusiound this on duck duck go Search intervirionfusion same text as in fauci email.