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posted ago by Narg ago by Narg +19 / -0

https://scitechdaily.com/affecting-up-to-216000-studies-popular-genetic-method-found-to-be-deeply-flawed/

. . . The field of paleogenomics, where we want to learn about ancient peoples and individuals such as Copper age Europeans, heavily relies on PCA. PCA is used to create a genetic map that positions the unknown sample alongside known reference samples. Thus far, the unknown samples have been assumed to be related to whichever reference population they overlap or lie closest to on the map.

However, Elhaik discovered that the unknown sample could be made to lie close to virtually any reference population just by changing the numbers and types of the reference samples (see illustration), generating practically endless historical versions, all mathematically “correct,” but only one may be biologically correct.

In the study, Elhaik has examined the twelve most common population genetic applications of PCA. He has used both simulated and real genetic data to show just how flexible PCA results can be. According to Elhaik, this flexibility means that conclusions based on PCA cannot be trusted since any change to the reference or test samples will produce different results.

Between 32,000 and 216,000 scientific articles in genetics alone have employed PCA for exploring and visualizing similarities and differences between individuals and populations and based their conclusions on these results.

“I believe these results must be re-evaluated,” says Elhaik.