Purpose
(Empty Backbone) Self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and luciferase.
Luciferase is not a virus, it is an enzyme which is a type of protein. What this is saying is that it is using a lentiviral plasmid. A plasmid is a ring of double stranded DNA that is often used for gene cloning. The desired genes are spliced into the plasmid which becomes a recombinant plasmid that can be replicated to produce large quantities of the desired genes.
No, I'm saying that they were splicing genes into the plasmid to go along with the HIV backbone, probably a spike protein along with the luciferase reporter.
I think that they were using the plasmids to produce altered viral DNA in order to create a modified virus. There is a diagram of the plasmid in your image, but it is too blurry to read.
Luciferase is not a virus, it is an enzyme which is a type of protein. What this is saying is that it is using a lentiviral plasmid. A plasmid is a ring of double stranded DNA that is often used for gene cloning. The desired genes are spliced into the plasmid which becomes a recombinant plasmid that can be replicated to produce large quantities of the desired genes.
Why are they using HIV plasmids? Can't these proteins infect us?
Are you saying they are splicing HIV genes into people?
No, I'm saying that they were splicing genes into the plasmid to go along with the HIV backbone, probably a spike protein along with the luciferase reporter.
I think that they were using the plasmids to produce altered viral DNA in order to create a modified virus. There is a diagram of the plasmid in your image, but it is too blurry to read.