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136
Primer Sequence CTCCCTTTGTTGTGTTGT (found in the WHO coronavirus PCR test document) is a sequence detected by PCR which designates a positive test result. It just happens the same sequence is found in humans and thus could find positive results in all. Is this the cause of excess false positives? (www.google.com)
posted 3 years ago by Ehjax78 3 years ago by Ehjax78 +138 / -2
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– DrJill 2 points 3 years ago +2 / -0

I used blastn and found many targets 12/17/21, 8:14 AM NCBI Blast:human file:///C:/Users/David/Documents/NCBI Blast_human.html 1/8 Job Title RID Program Database Query ID Description Molecule type Query Length BLAST ® » blastn suite » results for RID-VTA5XDMG016 Your search parameters were adjusted to search for a short input sequence. Your search is limited to records that include: Homo sapiens (taxid:9606) human ... VTA5XDMG016 Search expires on 12-18 22:11 pm BLASTN nt lcl|Query_45011 None ... nucleic acid 18 Descriptions COVID-19 Information Public health information (CDC) Research information (NIH) SARS-CoV-2 data (NCBI) Prevention and treatment information (HHS) Español Description ScientificName MaxScore TotalScore QueryCover Evalue Per.Ident Acc.Len Accession Homo sapiens chromosome 8,clone RP11-573J24, completesequence Homo sapiens 36.2 36.2 100% 0.23 100.00% 182712 AC018953.11 Homo sapiens BAC clone RP11-579E24 from 8, completesequence Homo sapiens 36.2 36.2 100% 0.23 100.00% 201146 AC069133.6 Human DNA sequence fromclone RP11-168P13 onchromosome 13, completesequence Homo sapiens 34.2 34.2 94% 0.91 100.00% 157175 AL162713.19 Homo sapiens 12 BAC RP11-131I18 (Roswell Park CancerInstitute Human BAC Library)complete sequence Homo sapiens 34.2 34.2 94% 0.91 100.00% 71430 AC063957.22 Homo sapiens pyruvatecarboxylase (PC), RefSeqGeneon chromosome 11; nuclear genefor mitochondrial product Homo sapiens 32.2 32.2 88% 3.6 100.00% 116851 NG_008319.1 Homo sapiens genomic DNA,chromosome 11q clone:RP11-15L8, complete sequences Homo sapiens 32.2 56.5 100% 3.6 100.00% 182769 AP003176.2 Homo sapiens chromosome 6clone CH17-331O20, completesequence Homo sapiens 30.2 54.5 88% 14 100.00% 204091 AC277811.1 PREDICTED: Homo sapiensmultivesicular body subunit 12B(MVB12B), transcript variant X3,mRNA Homo sapiens 30.2 30.2 83% 14 100.00% 3005 XM_024447711.1 Homo sapiens parkin RBR E3ubiquitin protein ligase (PRKN),RefSeqGene on chromosome 6 Homo sapiens 30.2 133 94% 14 100.00% 1387386 NG_008289.2 Homo sapiens chromosome 8clone CH17-499G4, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 231047 AC275565.1 Homo sapiens chromosome 8clone CH17-449G4, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 231223 AC270266.1 12/17/21, 8:14 AM NCBI Blast:human file:///C:/Users/David/Documents/NCBI Blast_human.html 2/8 Description ScientificName MaxScore TotalScore QueryCover Evalue Per.Ident Acc.Len Accession Homo sapiens neurotrophicreceptor tyrosine kinase 2(NTRK2), RefSeqGene onchromosome 9 Homo sapiens 30.2 78.8 94% 14 100.00% 365520 NG_012201.2 Homo sapiens isolate CHM13chromosome 18 Homo sapiens 30.2 3102 100% 14 100.00% 80542538 CP068260.2 Homo sapiens isolate CHM13chromosome 16 Homo sapiens 30.2 4283 100% 14 100.00% 96330374 CP068262.2 Homo sapiens isolate CHM13chromosome 15 Homo sapiens 30.2 4289 100% 14 100.00% 99753195 CP068263.2 Homo sapiens DNA,chromosome 18, nearly completegenome Homo sapiens 30.2 3073 100% 14 100.00% 77846715 AP023478.1 Homo sapiens DNA,chromosome 16, nearly completegenome Homo sapiens 30.2 4233 100% 14 100.00% 94690957 AP023476.1 Homo sapiens DNA,chromosome 15, nearly completegenome Homo sapiens 30.2 3751 100% 14 100.00% 95537968 AP023475.1 Homo sapiens chromosome 8clone VMRC59-81I15, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 121781 AC280315.1 Homo sapiens transcription factorDp-1 (TFDP1), RefSeqGene onchromosome 13 Homo sapiens 30.2 30.2 83% 14 100.00% 63786 NG_029909.1 Homo sapiens multivesicularbody subunit 12B (MVB12B),RefSeqGene on chromosome 9 Homo sapiens 30.2 107 94% 14 100.00% 187198 NG_029787.1 Homo sapiens zinc finger protein644 (ZNF644), RefSeqGene onchromosome 1 Homo sapiens 30.2 30.2 83% 14 100.00% 113958 NG_029760.1 Homo sapiens opioid bindingprotein/cell adhesion moleculelike (OPCML), RefSeqGene onchromosome 11 Homo sapiens 30.2 78.8 88% 14 100.00% 1124529 NG_012107.1 Homo sapiens FOSMID cloneABC7-42061100P2 fromchromosome unknown, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 41122 AC233314.1 Homo sapiens chromosome 16clone CTD-2537L13, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 186814 AC093516.5 Homo sapiens transcription factorDp-1 (TFDP1) gene, partial cds Homo sapiens 30.2 30.2 83% 14 100.00% 55319 AF550129.1 Homo sapiens 3 BAC RP11-432B6 (Roswell Park CancerInstitute Human BAC Library)complete sequence Homo sapiens 30.2 30.2 83% 14 100.00% 124701 AC079594.14 Human DNA sequence fromclone RP11-288A5 onchromosome 10, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 163778 AL391005.9 Human DNA sequence fromclone RP4-640E24 onchromosome 1q21.3-23.1,complete sequence Homo sapiens 30.2 54.5 94% 14 100.00% 102512 AL355862.16 Homo sapiens chromosome 3clone RP11-804H8, completesequence Homo sapiens 30.2 54.5 83% 14 100.00% 192324 AC096920.2 Homo sapiens chromosome 18,clone RP11-732E23, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 171501 AC104988.4 Homo sapiens chromosome 11clone PAC pDJ392a17, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 164155 AC000385.2 Homo sapiens chromosome 5clone RP11-510I6, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 162485 AC106806.2 Homo sapiens chromosome 18,clone RP11-9H16, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 173941 AC022701.6 12/17/21, 8:14 AM NCBI Blast:human file:///C:/Users/David/Documents/NCBI Blast_human.html 3/8 Description ScientificName MaxScore TotalScore QueryCover Evalue Per.Ident Acc.Len Accession Homo sapiens chromosome 18,clone RP11-603M14, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 168063 AC080051.6 Homo sapiens chromosome 18,clone RP11-636O21, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 198543 AC055820.7 Homo sapiens chromosome 11,clone RP11-348B14, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 160146 AC090284.6 Homo sapiens, clone RP11-25C13, complete sequence Homo sapiens 30.2 30.2 83% 14 100.00% 167292 AC016205.7 Homo sapiens chromosome 16clone RP11-2M17, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 176117 AC092352.2 Human DNA sequence fromclone RP11-30A1 onchromosome 9, completesequence Homo sapiens 30.2 54.5 94% 14 100.00% 124156 AL596132.7 Human DNA sequence fromclone RP11-243A2 onchromosome 1, completesequence Homo sapiens 30.2 54.5 83% 14 100.00% 164057 AL590138.7 Homo sapiens chromosome 11,clone RP11-4B7, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 174711 AC026639.6 Human DNA sequence fromclone RP11-85A1 onchromosome 10, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 123160 AL353719.10 Human DNA sequence fromclone RP11-230F18 onchromosome 13, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 184889 AL442125.13 Homo sapiens 12 BAC RP11-153K16 (Roswell Park CancerInstitute Human BAC Library)complete sequence Homo sapiens 30.2 30.2 83% 14 100.00% 157610 AC087235.13 Human DNA sequence fromclone RP11-127P7 onchromosome 6, completesequence Homo sapiens 30.2 54.5 88% 14 100.00% 108813 AL138718.17 Human DNA sequence fromclone RP5-924G13 onchromosome 1, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 126367 AL136109.11 Human DNA sequence fromclone RP1-119H20 onchromosome 6q25.3-27,complete sequence Homo sapiens 30.2 82.8 94% 14 100.00% 128710 AL078583.15 Homo sapiens chromosome 16clone RP11-212I21, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 193963 AC007336.6 Homo sapiens chromosome 8,clone RP11-303G3, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 174171 AC068587.16 Homo sapiens chromosome 15,clone RP11-385H1, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 203244 AC090983.13 Homo sapiens chromosome 8,clone RP11-400G5, completesequence Homo sapiens 30.2 54.5 88% 14 100.00% 166222 AC025056.5 Homo sapiens BAC clone RP11-425J3 from 2, completesequence Homo sapiens 30.2 30.2 83% 14 100.00% 105686 AC012072.2 Human chromosome 14 DNAsequence BAC R-889B13 oflibrary RPCI-11 fromchromosome 14 of Homo sapiens(Human), complete sequence Homo sapiens 30.2 54.5 94% 14 100.00% 184039 AL109767.7 Homo sapiens genomic DNA,chromosome 11, clone:RP11-419F8, complete sequence Homo sapiens 30.2 54.5 88% 14 100.00% 170006 AP005122.2 12/17/21, 8:14 AM NCBI Blast:human file:///C:/Users/David/Documents/NCBI Blast_human.html 4/8 Description ScientificName MaxScore TotalScore QueryCover Evalue Per.Ident Acc.Len Accession Human chromosome 14 DNAsequence BAC C-2200A16 oflibrary CalTech-D fromchromosome 14 of Homo sapiens(Human), complete sequence Homo sapiens 30.2 54.5 94% 14 100.00% 140073 AL132819.6 Homo sapiens chromosome 9,clone hRPK.494_N_15, completesequence Homo sapiens 30.2 107 94% 14 100.00% 210636 AC006443.1 Human 11p14.3 PAC clonepDJ939m16, complete sequence Homo sapiens 30.2 30.2 83% 14 100.00% 132867 AC004601.1 Human DNA sequence fromclone XXcos-4 on chromosome3, complete sequence Homo sapiens 30.2 30.2 83% 14 100.00% 34234 Z74019.1 Homo sapiens discs largeMAGUK scaffold protein 2(DLG2), transcript variant 1,mRNA Homo sapiens 28.2 28.2 100% 56 94.44% 7959 NM_001142699.3 Homo sapiens ATMserine/threonine kinase (ATM),transcript variant 2, mRNA Homo sapiens 28.2 28.2 77% 56 100.00% 12915 NM_000051.4 Homo sapiens discs largeMAGUK scaffold protein 2(DLG2), transcript variant 27,non-coding RNA Homo sapiens 28.2 28.2 100% 56 94.44% 7194 NR_165353.1 Homo sapiens discs largeMAGUK scaffold protein 2(DLG2), transcript variant 21,mRNA Homo sapiens 28.2 28.2 100% 56 94.44% 6040 NM_001377978.1 Homo sapiens discs largeMAGUK scaffold protein 2(DLG2), transcript variant 5,mRNA Homo sapiens 28.2 28.2 100% 56 94.44% 7728 NM_001206769.2 Homo sapiens discs largeMAGUK scaffold protein 2(DLG2), transcript variant 10,mRNA Homo sapiens 28.2 28.2 100% 56 94.44% 7710 NM_001377966.1 Homo sapiens discs largeMAGUK scaffold protein 2(DLG2), transcript variant 11,mRNA Homo sapiens 28.2 28.2 100% 56 94.44% 7674 NM_001377967.1 Homo sapiens discs largeMAGUK scaffold protein 2(DLG2), transcript variant 16,mRNA Homo sapiens 28.2 28.2 100% 56 94.44% 7580 NM_001377973.1 Homo sapiens discs largeMAGUK scaffold protein 2(DLG2), transcript variant 20,mRNA Homo sapiens 28.2 28.2 100% 56 94.44% 5925 NM_001377977.1 Homo sapiens discs largeMAGUK scaffold protein 2(DLG2), transcript variant 24,mRNA Homo sapiens 28.2 28.2 100% 56 94.44% 5935 NM_001377981.1 Homo sapiens discs largeMAGUK scaffold protein 2(DLG2), transcript variant 4,mRNA Homo sapiens 28.2 28.2 100% 56 94.44% 5986 NM_001142702.2 Homo sapiens discs largeMAGUK scaffold protein 2(DLG2), transcript variant 19,mRNA Homo sapiens 28.2 28.2 100% 56 94.44% 6078 NM_001377976.1 Homo sapiens discs largeMAGUK scaffold protein 2(DLG2), transcript variant 17,mRNA Homo sapiens 28.2 28.2 100% 56 94.44% 7135 NM_001377974.1 Homo sapiens discs largeMAGUK scaffold protein 2(DLG2), transcript variant 14,mRNA Homo sapiens 28.2 28.2 100% 56 94.44% 7518 NM_001377971.1 Homo sapiens discs largeMAGUK scaffold protein 2(DLG2), transcript variant 22,mRNA Homo sapiens 28.2 28.2 100% 56 94.44% 6028 NM_001377979.1 12/17/21, 8:14 AM NCBI Blast:human file:///C:/Users/David/Documents/NCBI Blast_human.html 5/8 Description ScientificName MaxScore TotalScore QueryCover Evalue Per.Ident Acc.Len Accession Homo sapiens discs largeMAGUK scaffold protein 2(DLG2), transcript variant 8,mRNA Homo sapiens 28.2 28.2 100% 56 94.44% 9469 NM_001351275.2 Homo sapiens discs largeMAGUK scaffold protein 2(DLG2), transcript variant 23,mRNA Homo sapiens 28.2 28.2 100% 56 94.44% 5968 NM_001377980.1 Homo sapiens discs largeMAGUK scaffold protein 2(DLG2), transcript variant 13,mRNA Homo sapiens 28.2 28.2 100% 56 94.44% 7572 NM_001377970.1 Homo sapiens discs largeMAGUK scaffold protein 2(DLG2), transcript variant 12,mRNA Homo sapiens 28.2 28.2 100% 56 94.44% 7710 NM_001377968.1 Homo sapiens discs largeMAGUK scaffold protein 2(DLG2), transcript variant 15,mRNA Homo sapiens 28.2 28.2 100% 56 94.44% 7291 NM_001377972.1 Homo sapiens discs largeMAGUK scaffold protein 2(DLG2), transcript variant 25,mRNA Homo sapiens 28.2 28.2 100% 56 94.44% 5809 NM_001377982.1 Homo sapiens kazrin, pe

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– CokeOrPepe 1 point 3 years ago +1 / -0

Can you format this a little better? kthx

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– DrJill 1 point 2 years ago +1 / -0

email me for format

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– Ehjax78 [S] 0 points 3 years ago +1 / -1

I'm curious. What's Blastn

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– DrJill 1 point 2 years ago +1 / -0

nucleotide search in genbank database

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