I'm not writing a dissertation in response to a comment on a forum, smart guy. If that's your expectation in response to your claims (for which we notice that you yourself did not provide substantiary evidence. Hypocrisy, thou name art MAG768720), then you're destined to live in your own echo chamber.

Vero cells are used for culturing many viruses that are tropic for monkeys and kidneys, but other viruses that are tropic for multiple species or organs are grown in those cell cultures. Vero is not the most commonly used either. ATCC had a comprehensive list of those cell lines for further research on your own time, should you be interested. Methinks you are not, because you are drowning in the Koolaid of your own ignorance, but hey, you do you man.

Viruses are regularly purified by density gradient centrifugation. Next.

As far as Lanka goes, he just demonstrated that some cell culture components are capable of causing CPE at certain concentrations, given enough time. There's a reason laboratories titer their media components to ensure they're not making cells sick on their own before testing anything else on them. All conditions have to be the same on an experiment, save the single condition being tested. Plus or minus virus.

If he wanted to demonstrate that viruses in culture are not causing CPE, he would need to culture a sample in their correct cell line for a positive group, and then culture cells regularly without the virus, or if he wanted to be extra smart, coincubate sonicated cells from samples from uninfected individuals. Monitor CPE. Or, if one is limited access to human samples, or concerned with narrowing down which components of cell culture are causing CPE, you can just take the supernatants from first cultures and use them to infect a secondary cell culture to observe CPE. This would demonstrate that an extracellular component was capable of causing CPE in the positive vs negative control. He could also test intracellular components by sonicating the cell layer of the first set of experimental groups, and using the cell lysates to infect and observe CPE over time. This is pretty basic virological and cell culture techniques. You can also run western blots, immunofluorescent microscopy, electron microscopy, PCR or sequencing to further narrow down what is causing the issue. There is sufficient evidence that viruses are real and they induce pathological effects that can be reliably documented.

I'm not writing a dissertation in response to a comment on a forum, smart guy. If that's your expectation in response to your claims (for which we notice that you yourself did not provide substantiary evidence. Hypocrisy, thou name art MAG768720), then you're destined to live in your own echo chamber.

Vero cells are used for culturing many viruses that are tropic for humans and kidneys, but other viruses that are tropic for multiple species or organs are grown in those cell cultures. ATCC had a comprehensive list of those cell lines for further research on your own time, should you be interested. Methinks you are not, because you are drowning in the Koolaid of your own ignorance, but hey, you do you man.

Viruses are regularly purified by density gradient centrifugation. Next.

As far as Lanka goes, he just demonstrated that some cell culture components are capable of causing CPE at certain concentrations, given enough time. There's a reason laboratories titer their media components to ensure they're not making cells sick on their own before testing anything else on them. All conditions have to be the same on an experiment, save the single condition being tested. Plus or minus virus.

If he wanted to demonstrate that viruses in culture are not causing CPE, he would need to culture a sample in their correct cell line for a positive group, and then culture cells regularly without the virus, or if he wanted to be extra smart, coincubate sonicated cells from samples from uninfected individuals. Monitor CPE. Or, if one is limited access to human samples, or concerned with narrowing down which components of cell culture are causing CPE, you can just take the supernatants from first cultures and use them to infect a secondary cell culture to observe CPE. This would demonstrate that an extracellular component was capable of causing CPE in the positive vs negative control. He could also test intracellular components by sonicating the cell layer of the first set of experimental groups, and using the cell lysates to infect and observe CPE over time. This is pretty basic virological and cell culture techniques. You can also run western blots, immunofluorescent microscopy, electron microscopy, PCR or sequencing to further narrow down what is causing the issue. There is sufficient evidence that viruses are real and they induce pathological effects that can be reliably documented.