Please tell me where I called it an "awesome paper". I am just sharing what I find from my sources and on this particular one I get a shitty trash replying back after I explicitly said in the first comment "I am not an expert".

A T7-based pET-26 plasmid with a N-terminal His8 tag followed by a TEV cleavage site was used

Dont know what it means and never professed I did.

to express codon optimized versions

Codon - A sequence of three adjacent nucleotides constituting the genetic code that determines the insertion of a specific amino acid in a polypeptide chain during protein synthesis or the signal to stop protein synthesis.

Yes they are playing around with the amino acid proteins in here.

Coronaviruses (CoV) form large multicomponent RNA replication-transcription complexes containing a core RNA synthesis machine made of the nsp12 RdRP protein with one nsp7 and two nsp8 proteins as essential subunits required for activity. We show that assembly of this complex can be accelerated 5-fold by preincubation of nsp12 with nsp8 and further optimized with the use of a novel nsp8L7 heterodimer fusion protein construct.

All proteins used in this study were expressed in Escherichia coli using T7 promoters and purified through metal-affinity, ion-exchange, and size-exclusion chromatography (Fig. 2A).

So they are genetically modifying proteins in E Coli to mimic those of SARS-Cov-2 to understand their mechanism. This very common and much easier way to experiment than with an actual virus that includes all the extra biosafety regulations and costs. In the end you still just want to understand the pieces that make the virus work.

The virus is not a flu, the genes have been sequenced and it was even shown that Modern a had patented sequences of its genes back in 2017. These patented genes do not show up naturally in any form of life prior to the pandemic. This is interesting if you take into account of Monsanto GMO patent court cases in the past, it means Moderna owns SARS-Cov-2 and all the damage that comes with it. You can prove this for yourself with BLAST, I will go find the past article and send if you want.

Please tell me where I called it an "awesome paper". I am just sharing what I find from my sources and on this particular one I get a shitty trash replying back after I explicitly said in the first comment "I am not an expert".

A T7-based pET-26 plasmid with a N-terminal His8 tag followed by a TEV cleavage site was used

Dont know what it means and never professed I did.

to express codon optimized versions

Codon - A sequence of three adjacent nucleotides constituting the genetic code that determines the insertion of a specific amino acid in a polypeptide chain during protein synthesis or the signal to stop protein synthesis.

Yes they are playing around with the amino acid proteins in here.

Coronaviruses (CoV) form large multicomponent RNA replication-transcription complexes containing a core RNA synthesis machine made of the nsp12 RdRP protein with one nsp7 and two nsp8 proteins as essential subunits required for activity. We show that assembly of this complex can be accelerated 5-fold by preincubation of nsp12 with nsp8 and further optimized with the use of a novel nsp8L7 heterodimer fusion protein construct.

All proteins used in this study were expressed in Escherichia coli using T7 promoters and purified through metal-affinity, ion-exchange, and size-exclusion chromatography (Fig. 2A).

So they are genetically modifying proteins in E Coli to mimic those of SARS-Cov-2 to understand their mechanism. This very common and much easier way to experiment than with an actual virus that includes all the extra biosafety regulations and costs. In the end you still just want to understand the pieces that make the virus work.

The virus is not a flu, the genes have been sequenced and it was even shown that Modern a had patented sequences of its genes back in 2017 which is interesting if you take into account of Monsanto GMO patent court cases in the past, it means Moderna owns SARS-Cov-2 and all the damage that comes with it. You can prove this for yourself with BLAST, I will go find the past article and send if you want.

Please tell me where I called it an "awesome paper". I am just sharing what I find from my sources and on this particular one I get a shitty trash replying back after I explicitly said in the first comment "I am not an expert".

A T7-based pET-26 plasmid with a N-terminal His8 tag followed by a TEV cleavage site was used

Dont know what it means and never professed I did.

to express codon optimized versions

Codon - A sequence of three adjacent nucleotides constituting the genetic code that determines the insertion of a specific amino acid in a polypeptide chain during protein synthesis or the signal to stop protein synthesis.

Yes they are playing around with the amino acid proteins in here.

Coronaviruses (CoV) form large multicomponent RNA replication-transcription complexes containing a core RNA synthesis machine made of the nsp12 RdRP protein with one nsp7 and two nsp8 proteins as essential subunits required for activity. We show that assembly of this complex can be accelerated 5-fold by preincubation of nsp12 with nsp8 and further optimized with the use of a novel nsp8L7 heterodimer fusion protein construct.

All proteins used in this study were expressed in Escherichia coli using T7 promoters and purified through metal-affinity, ion-exchange, and size-exclusion chromatography (Fig. 2A).

So they are genetically modifying proteins in E Coli to mimic those of SARS-Cov-2 to understand their mechanism. This very common and much easier way to experiment than with an actual virus that includes all the extra biosafety regulations and costs. In the end you still just want to understand the pieces that make the virus work.

Please tell me where I called it an "awesome paper". I am just sharing what I find from my sources and on this particular one I get a shitty trash replying back after I explicitly said in the first comment "I am not an expert".

A T7-based pET-26 plasmid with a N-terminal His8 tag followed by a TEV cleavage site was used

Dont know what it means and never professed I did.

to express codon optimized versions

Codon - A sequence of three adjacent nucleotides constituting the genetic code that determines the insertion of a specific amino acid in a polypeptide chain during protein synthesis or the signal to stop protein synthesis.

Yes they are playing around with the amino acid proteins in here.

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