(motivated by finding out that NSW is cycling 45 times)(for the US boiz i think your highest cycle rate was 30-35 and dropped to 20ish the day biden was installed)
rough draft: running 45 cycles duplicates the DNA test sample 35 trillion fkn times with a chance to mutate each duplication they heat the DNA until it breaks into 2 halves then grow the missing half back using enzymes "Taq polymerase" giving you 2 full strands (cloning), this repeats exponentially each cycle, 1 2 4 8 16 32 64 ect ect 45 cycles = 2^45 = 35,000,000,000,000 you can take any rate of mutation and apply it to that number and it doesn't matter how many matches to the sequence of covid you need for a positive test result at that point, you'll get them.
And they don't even use the full genetic code of the virus, they're just using a portion of it, the reasoning is it's too expensive and takes too long to analyse during an emergency.
PCR tests are meant to be used around 5-10 cycles and that's just to find out if it's likely that a virus might be present in the test sample and is mainly just for doing research, it's not even made for diagnosis of infections. (coming from the nobel prize winner who invented it)
They determine a positive result by a set number of matches to the SARS COV-2 sequence out of the 35 trillion samples with a huge chuck of them being mutated ones, 0.5% would be 1.75 trillion mutated samples, (can't find required number of matches best i could find is that the parameters are set by the manufacturers)
I have heard that the sequence being used was just sent over from China in an email when the outbreak first started and that's been the only global standard sequence since it started. (could dig up source, further investigation needed)
He tells a story about how it made him believe the Buddhist idea that "everything contains everything" (or how ever it goes) because you can create every sequence in the human body in 1 billion mutations if they were all unique and all mutated, just 30 cycles. 30 cycles = 1.07 billion samples
45 = 35 trillion samples. What are the odds that there is a shitload of matching sequences in there. Pretty damn high.
Copy pasta from discussion with my best friend, sorry if some parts are a bit slang.