Request for information from anons educated in PCR test.
🧐 Research Wanted 🤔
My wife is required to submit to weekly testing for the coof. I asked the testing facility what Cycle Threshold they use for their tests. Here is the response:
**Our test does not use cycle thresholds (Ct). Ct is used for a qPCR test method, whereas we perform rRT-PCR. Our detection is given by flourescently tagged molecules that generate Median Fluorescent Intensity (MFI) based on the viral load of the collected sample. We test for multiple genes within the SARS-CoV-2 genome and have a different threshold for each. I hope that answers your question. **
I 'think' I understand but perhaps there is an anon in here that can give a brief explanation regarding accuracy etc.
Can't say it better than this: http://www.differencebetween.net/science/difference-between-rt-pcr-and-qpcr/
Reverse Transcription PCR (RT-PCR) versus Real Time PCR (qPCR).
Other things you need to check for are the actual protocol that is being used. There are many protocols that used a mix of influenza, human cells, and a sample thought to include COVID19. These tests are the exact ones that the CDC is trying to phase out but is still allowing until December - meaning that false positives have been rampant because the test is picking up almost anything without even discussing the cycle threshold issue.
1.QPCR and RT-PCR are both terms used in biotechnology and utilized for the production of multiple copies of DNA. 2.RT-PCR is used to amplify the reversed transcription of the DNA code; QPCR measures the amplification. 3.RT-PCR is for amplification, while qPCR is for quantification. 4.QPCR is quantitative in nature, while RT-PCR is not.