Request for information from anons educated in PCR test.
🧐 Research Wanted 🤔
My wife is required to submit to weekly testing for the coof. I asked the testing facility what Cycle Threshold they use for their tests. Here is the response:
**Our test does not use cycle thresholds (Ct). Ct is used for a qPCR test method, whereas we perform rRT-PCR. Our detection is given by flourescently tagged molecules that generate Median Fluorescent Intensity (MFI) based on the viral load of the collected sample. We test for multiple genes within the SARS-CoV-2 genome and have a different threshold for each. I hope that answers your question. **
I 'think' I understand but perhaps there is an anon in here that can give a brief explanation regarding accuracy etc.
I'm still stuck back at "we don't have the genetic sequence of sars-cov-2" I haven't be able to find it. No sequence, no primers* for a PCR test. Last I read, CDC has proprietary primers for the PCR test, and they're not telling us what they are. EVERYONE who is running the PCR test has to get the priming agents from CDC (no conflict of interest there - kek) So not only were the (40) PCR cycles too high, we don't actually know what the primers are finding in the sample.
We always ran a positive control, and two negative controls: pure water source, and reagents only without sample. If you run those controls, you will see artificial ghosting/false positive. (research and development background, not clinical). I'm thinking the primers probably were designed to pick up any of the 7 common corona viruses...and possibly some influenza stuff too. Who knows? Only the crooks at CDC. This whole mess has been planned/executed with high level military precision by evil satanists IMO. So yes, the 'new' tests are probably bogus too. SHOW US THE PRIMERS so we can find what they attach to!!!
*Note: PCR test requires two primers to attach to (both ends of) a very specific region of the DNA. designing primers is not always simple thing. They must be exact, and only target a unique piece of the organism and not match up with other stuff floating in the sample.