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New Pfizer Docs Up! (phmpt.org) 🧐 Research Wanted 🤔

posted 2 years ago by DagnyDocket 2 years ago by DagnyDocket +458 / -0

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– DagnyDocket [S] 44 points 2 years ago +44 / -0

Are we still conspiracy theorists for talking about Luciferase and that it glows/bioluminescence?

EXPRESSION OF LUCIFERASE-ENCODING MODRNA AFTER I.M. APPLICATION OF GMP- READY ACUITAS LIPID NANOPARTICLE FORMULATION

https://phmpt.org/wp-content/uploads/2022/03/125742_S1_M4_4223_R-20-0072.pdf

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– forgotmypassword123 3 points 2 years ago +3 / -0

4.4 Materials

Luciferin Substrate for in vivo luciferase imaging 122799-10 150 mg/kg Perkin-Elmer

QuantiLum recombinant luciferase Positive control E1701 100 ng/µL Promega ** volumetric, 0.1 M HCl 2104-50 ml N/A Sigma-Aldrich

RPMI1640 medium Cell culture medium 61870 N/A Gibco

Anti-firefly luciferase antibody (mAb21), Assay control ELISA ab64564 1:1,000 Abcam Mouse IgG isotype Assay control ELISA 0107-08 1:100 as starting dilution Southern Biotech

Goat anti-mouse IgG HRP Secondary antibody ELISA 115-035- 071 1:15,000 (if stored in 50% glycerol 1:7,500) Jackson WTF?

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