Revelation 13:15 And he had power to give life unto the image of the beast, that the image of the beast should both speak, and cause that as many as would not worship the image of the beast should be killed. 16 And he causeth all, both small and great, rich and poor, free and bond, to receive a mark in their right hand, or in their foreheads:

The mark of the beast is not a one time thing. The beast system runs the world and we're all in it.

Revelation 14:1 And I looked, and, lo, a Lamb stood on the mount Sion, and with him an hundred forty and four thousand, having his Father's name written in their foreheads.

144,000 is 12 x 12 x 1000, it is a symbol of perfect government. The 144,000 are governed by the Father, they have disguarded the beasts economy, they don't bend to the beasts way of business.

It is shocking to think that it is either one or the other, it's either the beast or the Father.

Jesus Christ makes all things new(virgin), the 144,000 are virgins, this is a spiritual concept.

Revelation 14:4 These are they which were not defiled with women; for they are virgins. These are they which follow the Lamb whithersoever he goeth. These were redeemed from among men, being the firstfruits unto God and to the Lamb.

In order to defeat the mark of the beast you "follow the Lamb withersoever he goeth".

Revelation 15:2 And I saw as it were a sea of glass mingled with fire: and them that had gotten the victory over the beast, and over his image, and over his mark, and over the number of his name, stand on the sea of glass, having the harps of God.

The sea of glass mingled with fire is peace, when you've gotten the victory over the mark, you become new, a virgin, and the peace of God rules you.