I am not an expert, but I think they are blowing smoke up your ass. Here is an explanation of differences in testing procedures:

https://www.enzolifesciences.com/science-center/technotes/2017/march/what-are-the-differences-between-pcr-rt-pcr-qpcr-and-rt-qpcr?/

My understanding is this: The original PCR methodology was used on DNA, since that is what Mullis was using it for. Over time, somebody came up with a method to take RNA material and supposedly "reverse it" back into DNA. Once they do that, they call it cDNA, but the PCR process from there is the same.

The tagged molecules are used in both cases, when someone is (fraudulently) attempting to use it for "diagnosis." Remember, Mullis said PCR canNOT be used to diagnose. So, what they are doing is using PCR to amplify (i.e. multiply) the amount of material, and then from there, adding to that a technique where they CLAIM that if the flourescent activity is "high enough" then they will conclude "positive" for the test.

But it is a SCAM. The problem is, at this point there are multiple mixed issues that need to be unraveled in order to see the scam.

Multiple problems:

• They are starting with RNA, not DNA
• DNA = a complete set of instructions for how to "build" the various parts of an organism
• RNA = molecules that carry out the instructions of the DNA
• They are related, but not the same
• RNA fragments in one organism can be identical to RNA fragments in another organism
• SARS-CoV-2 has never been isolated and purified
• Instead, RNA fragments supposedly from SARS-CoV-2 have been claimed to be part of the virus, but there is no sample of DNA from the virus anywhere in the world in any laboroatory, so it is not proven (it is done via computer modeling)
• PCR is still a method of amplification, or replicating material, no matter what they are claiming

Also note: They cannot perform a medical procedure on you UNLESS they get fully informed consent from you (or in this case, from your wife), and they can only do that if they provide FULL INFORMATION. So, asking questions until you get complete answers that you can reasonably understand is your right, and they cannot coerce you into doing the procedure without informed consent.

My response would be something like this:

Thank you for your response. However, your response has raised more questions. Please provide me with the following information:

1. You state, "Our detection is ... based on the viral load of the collected sample." How is the viral load obtained, upon which you perform your detection? Do you take a nasal swab and only use that material? Or, do you perform some additional process that increases (amplifies or multiples) the amount of material you have available upon which you can perform your method?

2. You state that you "... have a different threshold for each [genome]." What do you mean by the word "threshold" if, as you stated, you do not use cycle thresholds? How are these thresholds determined? What scientific studies are these thresholds based on?

3. It is my understanding that PCR (Polymerase Chain Reaction) is a mechanical process used to multiply ("amplify") a sample of material into more of that material, so that it can be used for various research. The inventor of the technique, Dr. Kary Mullis, stated, "It can make a whole lot of something out of a little bit of something, but it cannot tell you if what you started with can make you sick." He invented the PCR method. He should know what it is, what it does, and what it cannot do. It is my understanding that RT-PCR is the same or a variation of this same technique, but starting with RNA rather than DNA. How does your technique differ, fundamentally, from the original PCR methodology that Dr. Mullis invented?

4. How do you determine a "positive" or "negative" diagnosis or other outcome in your results, once you have completed your procedure on a sample? Please provide me with any scientific studies that show that such a diagnosis is scientifically valid.

5. Please provide me with a list of the specific gene sequences you utilize in your testing and/or procedures.

6. Are any of these gene sequences RNA fragments?

7. Are any of these gene sequences identical to any human chromosome genes or RNA?

As I am sure you are well aware, I must be provided with full information about a medical procedure in order to give my informed consent to that procedure. No medical procedure can be mandated without informed consent.

Thank you for your prompt response to my questions.