Request for information from anons educated in PCR test.
🧐 Research Wanted 🤔
My wife is required to submit to weekly testing for the coof. I asked the testing facility what Cycle Threshold they use for their tests. Here is the response:
**Our test does not use cycle thresholds (Ct). Ct is used for a qPCR test method, whereas we perform rRT-PCR. Our detection is given by flourescently tagged molecules that generate Median Fluorescent Intensity (MFI) based on the viral load of the collected sample. We test for multiple genes within the SARS-CoV-2 genome and have a different threshold for each. I hope that answers your question. **
I 'think' I understand but perhaps there is an anon in here that can give a brief explanation regarding accuracy etc.
That is not correct. Nobody has isolated AND purified it so that SARS-CoV-2 is the ONLY thing being looked at. All those so-called "isloated" samples are called isolated because they come from one patient, but they are mixed in with kidney cells and other material that are not SARS-CoV-2. So, there is no way to say you ONLY have the virus to look at. That is the point of "isolated AND purified."
You want to separate it from everything else so that you are ONLY looking at and analyzing SARS-CoV-2, and nothing else. This has NEVER been done. Therefore, there is NO WAY to know that what you are analyzing is the virus versus the other things mixed in with it.
Then how did they make a vaccine for it Mr. Smarty Pants! /s