Request for information from anons educated in PCR test.
🧐 Research Wanted 🤔
My wife is required to submit to weekly testing for the coof. I asked the testing facility what Cycle Threshold they use for their tests. Here is the response:
**Our test does not use cycle thresholds (Ct). Ct is used for a qPCR test method, whereas we perform rRT-PCR. Our detection is given by flourescently tagged molecules that generate Median Fluorescent Intensity (MFI) based on the viral load of the collected sample. We test for multiple genes within the SARS-CoV-2 genome and have a different threshold for each. I hope that answers your question. **
I 'think' I understand but perhaps there is an anon in here that can give a brief explanation regarding accuracy etc.
BTW, Slyver --
Have you read through this info on this topic?
https://rightsfreedoms.wordpress.com/2021/07/19/the-scam-has-been-confirmed-pcr-does-not-detect-sars-cov-2-but-endogenous-gene-sequences/
Would like to know your feedback.
I don't have time atm, but I will read it later.
I have been meaning to do a deep dive into the primers and how they might match with other organisms or viruses (H1N1, other coronaviruses, etc.). I know that the N-proteins that some of the PRC tests look at (per the CDC guidelines) are almost the same size across the board of these related RNA viruses. Close enough that on a gel they will easily be confused, especially when one "errs on the side of caution." The same errors would occur with the rRT-PCR if the primers were close enough and the cycling high enough. If there is a close enough primer match with some of these other viruses it could be a smoking gun. I haven't seen anyone else do that analysis.
I think you will find the article interesting. I realize it takes more than 30 seconds to get through it. ;-)
Catch you later.