Yes and this is all the more impactful because of how Trump DIDN'T phrase it.

It's the way he didn't phrase it that IMO makes it a direct Q reference:

1. In context, the uses the words "the FBI and" before "Justice."

Having read thousands of his messages...IMHO in the flow of mid-sentence, he would be way more likely to have written "...the FBI and DOJ..." He'd just used a three-letter acronym. Why suddenly change it to full words—but then not even put the full words Department of Justice, but rather SHORTEN it to Justice? If he'd wanted to shorten it, he'd have just said DOJ, as he did with FBI.

2. Also, the word Justice has enough other meanings that it's rarely used alone to refer to the Department of Justice (except among insiders, wonks, or officials). The acronym DOJ or the full name are far more common in normal conversation.

Then:

3. He doesn't just write "...the FBI and Justice illegally colluding..."

He puts "Justice" in Quotes. With a capital Q.

With the ingenious plausible deniability of the quotes merely mocking the idea of the DOJ being about "justice" at all.

That would make it merely another bizarre coincidence that exactly five years earlier Q had posted that word, by itself, with capital J.

This one is so obviously a Q reference. It would've been even cooler if Trump had posted it at 3:09, but you can't have everything I guess.