the cycles used on the test - have a threshold before exponentially rising - which are interpreted as a positive - at 20 cycles the threshold in the exponential phase is reached by 30 cycles the plateau (aka POSITIVE RESULT) is reached - and the direction for the USEFUL IDIOTS administering these tests - were instructed to do 35-40 cycles. This INSURED A POSITIVE REGARDLESS OF WHAT WAS IN THE SAMPLE....wake up people - do some research.