Ok, so I think you misunderstand me. I am not on any particular bandwagon. And before you compare me again to a flat earther, which is just ad hominem, I asked you what do you think about mixing a sample of a person's lung lavage with animal cells or sick human cells, antibiotics etc. in order to 'isolate it'. you must admit this is counterintuitive, and there are methodologies that do not do this. Also, I have seen methodologies that add a wide mixture of these foreign cells.
You claim that in your lab, only one type of cell is grown for each specific virus (well of course the 'host cells are replicated for this purpose, this is why the human cancer cells have lasted all these years, and why that particular cancer was chosen, because it is particularly virulent and therefore replicating rapidly). So that's interesting. This is why I asked you to elaborate, because it is hard to find this type of information.
As for my request for sauce - as you admitted previously, there is a lot of information crowding into this space, and it is confusing, even for someone trained to read methodologies and critique them. So, I again re-iterate what are your sources apart from, but as well as your personal experience? So, I am hoping that you can get to a computer and reveal to us the literature you are using.
I wasn't comparing you to a flat earther, there was no ad hominem attack. I took your comment to mean that you hadn't made up your mind what to believe and were asking my opinion, and I was simply pointing out that the many people I see post on this site (not you) that insist that no virus has ever been isolated are ridiculously misinformed and have the tendency to believe every wild ass thing they see on a youtube video and it's impossible to change their mind. The point of my comment is that you should not believe these people, they seem to read something on the internet and just believe it without any knowledge or understanding of whether it comes from an intelligent source or a complete moron.
In our labs, we focus on high-throughput screening so that we can test thousands of experimental compounds per year in vitro (cell culture). Less than one percent of those compounds are selected to go into a mouse infection model for further study. We don't do much isolation of viruses, we obtain virus isolates from other sources for our work, and so I am not familiar with the current methods being used for such work, but clearly we couldn't get all our virus isolates if they didn't exist. I can't comment on your question about some of the procedures you've read about on that topic.
For each virus, we use the cell line that the virus grows best in for us to perform our testing. High throughput screening, we just want to know if a compound shows any activity against the virus or not. We're generally not looking for more detail than that, so I can't answer all of your questions. Seed the cells on a tissue culture plate, and 24 hours later add the drugs to the cells and then infect the cells with the virus and observe what happens.
My source is simply 30 years of working in virology labs doing the sort of testing I've described. I'm sorry if that's not the type of source you're looking for. I read papers online, especially since all the COVID bullshit started, and those are available to anyone who wants to search for them. I don't keep a database of everything I read, and I gave you the few links that I had bookmarked and commented on some of the others from memory (which is not always perfect).
Ok, so I think you misunderstand me. I am not on any particular bandwagon. And before you compare me again to a flat earther, which is just ad hominem, I asked you what do you think about mixing a sample of a person's lung lavage with animal cells or sick human cells, antibiotics etc. in order to 'isolate it'. you must admit this is counterintuitive, and there are methodologies that do not do this. Also, I have seen methodologies that add a wide mixture of these foreign cells.
You claim that in your lab, only one type of cell is grown for each specific virus (well of course the 'host cells are replicated for this purpose, this is why the human cancer cells have lasted all these years, and why that particular cancer was chosen, because it is particularly virulent and therefore replicating rapidly). So that's interesting. This is why I asked you to elaborate, because it is hard to find this type of information.
As for my request for sauce - as you admitted previously, there is a lot of information crowding into this space, and it is confusing, even for someone trained to read methodologies and critique them. So, I again re-iterate what are your sources apart from, but as well as your personal experience? So, I am hoping that you can get to a computer and reveal to us the literature you are using.
I wasn't comparing you to a flat earther, there was no ad hominem attack. I took your comment to mean that you hadn't made up your mind what to believe and were asking my opinion, and I was simply pointing out that the many people I see post on this site (not you) that insist that no virus has ever been isolated are ridiculously misinformed and have the tendency to believe every wild ass thing they see on a youtube video and it's impossible to change their mind. The point of my comment is that you should not believe these people, they seem to read something on the internet and just believe it without any knowledge or understanding of whether it comes from an intelligent source or a complete moron.
In our labs, we focus on high-throughput screening so that we can test thousands of experimental compounds per year in vitro (cell culture). Less than one percent of those compounds are selected to go into a mouse infection model for further study. We don't do much isolation of viruses, we obtain virus isolates from other sources for our work, and so I am not familiar with the current methods being used for such work, but clearly we couldn't get all our virus isolates if they didn't exist. I can't comment on your question about some of the procedures you've read about on that topic.
For each virus, we use the cell line that the virus grows best in for us to perform our testing. High throughput screening, we just want to know if a compound shows any activity against the virus or not. We're generally not looking for more detail than that, so I can't answer all of your questions. Seed the cells on a tissue culture plate, and 24 hours later add the drugs to the cells and then infect the cells with the virus and observe what happens.
My source is simply 30 years of working in virology labs doing the sort of testing I've described. I'm sorry if that's not the type of source you're looking for. I read papers online, especially since all the COVID bullshit started, and those are available to anyone who wants to search for them. I don't keep a database of everything I read, and I gave you the few links that I had bookmarked and commented on some of the others from memory (which is not always perfect).