You don't even know what a paper is supposed to look like.
No "Materials and Methods" section?
Of course not, because it is not a paper. But you don't understand that, do you?
The first sentence:
Positive-strand RNA viruses replicate their genomes using virally encoded RNA-dependent RNA polymerases (RdRP) with a common active-site structure and closure mechanism upon which replication speed and fidelity can evolve to optimize virus fitness.
That is a supposed statement of fact. Notice, it has NO REFERENCE to back it up.
The same is true of every sentence in the paragraph. No references whatsoever. No research to back it up. Just baseless statements of opinion, not science.
This is not a research paper. It is propaganda.
And you don't know how to tell the difference, which is why you are gullible in believing someone's assertions with zero proof.
Holy shit idiot do you know what an abstract is? Learn about how published research papers are written you fucking moron.
It literally says full text link in the link I already gave. An abstract is just a summary of their findings. Its the appetizer thats tasted if someone wants to read further.
A T7-based pET-26 plasmid with a N-terminal His8 tag followed by a TEV cleavage site was used
Do you know what that means? Explain.
to express codon optimized versions
Do you know what that means? Explain.
of the Wuhan-1 strain SARS-CoV-2 proteins
Why the proteins, and NOT the "virus?"
Do you know where those proteins came from? Do you know what was MIXED with what, and which proteins were being observed?
Right off the bat, we know they were NOT looking at a "virus," but rather at proteins.
So ... WHICH proteins would be an important thing to know.
The nsp8L7 fusion proteins were made by adding ...
Making proteins, not studying a virus.
They are supposedly (according to you) studying RNA virus, using PCR, and yet they are actually looking at proteins -- that were made from a MIXTURE of material, none of which has anything at all to do with a "virus."
RNA is in a lot of things. In this case, bacteria.
See the problem?
It is all ASSUMED to be somehow related to RNA virus -- and that ain't science.
All proteins were expressed in E. coli BL21(DE3) CodonPlus cells (Agilent Technologies) and grown
Mixing with E. Coli is not studying a virus.
It is studying a mixture of genetic material.
A MIXTURE.
Harvested cells were lysed [broken apart] ... using a ... microfluidizer [machine] ... in a buffer containing [chemicals] ...
So ...
They are taking proteins from E. Coli -- NOT FROM SARS-CoV-2 VIRUS.
E Coli is bacteria, not virus.
They claim they can CREATE something "SIMILAR TO" SARS-CoV-2 from this method.
Notice ... they are NOT STARTING WITH A SARS VIRUS.
Why jump through all these hoops? Why not just get a sample of SARS-CoV-2 virus and go from there?
Oh ... because they CAN'T. More than 100 agencies and laboratories around the world ALL have stated on the record that ... NO SUCH SAMPLE EXISTS, ANYWHERE IN THE WORLD.
THAT ... is why they jump through these ridiculous hoops.
Then, they break what they have apart by ADDING CHEMICALS.
Then, they centrifuge and claim they have something worth looking at.
Finally, they draw conclusions about "RNA Virus" based on this MIXTURE of materials that includes CHEMICALS that would not exist inside the body ... if the virus existed.
And they do it all with bacteria, no virus even included.
You are claiming this has some sort of great "proof" related to genomic sequencing and SARS and Covid (ultimately).
In reality, it has nothing to do with the science of so-called virus.
Proteins from bacteria and other materials. So what?
Please tell me where I called it an "awesome paper". I am just sharing what I find from my sources and on this particular one I get a shitty trash replying back after I explicitly said in the first comment "I am not an expert".
A T7-based pET-26 plasmid with a N-terminal His8 tag followed by a TEV cleavage site was used
Dont know what it means and never professed I did.
to express codon optimized versions
Codon - A sequence of three adjacent nucleotides constituting the genetic code that determines the insertion of a specific amino acid in a polypeptide chain during protein synthesis or the signal to stop protein synthesis.
Yes they are playing around with the amino acid proteins in here.
Coronaviruses (CoV) form large multicomponent RNA replication-transcription complexes containing a core RNA synthesis machine made of the nsp12 RdRP protein with one nsp7 and two nsp8 proteins as essential subunits required for activity. We show that assembly of this complex can be accelerated 5-fold by preincubation of nsp12 with nsp8 and further optimized with the use of a novel nsp8L7 heterodimer fusion protein construct.
All proteins used in this study were expressed in Escherichia coli using T7 promoters and purified through metal-affinity, ion-exchange, and size-exclusion chromatography (Fig. 2A).
So they are genetically modifying proteins in E Coli to mimic those of SARS-Cov-2 to understand their mechanism. This very common and much easier way to experiment than with an actual virus that includes all the extra biosafety regulations and costs. In the end you still just want to understand the pieces that make the virus work.
The virus is not a flu, the genes have been sequenced and it was even shown that Modern a had patented sequences of its genes back in 2017. These patented genes do not show up naturally in any form of life prior to the pandemic. This is interesting if you take into account of Monsanto GMO patent court cases in the past, it means Moderna owns SARS-Cov-2 and all the damage that comes with it. You can prove this for yourself with BLAST, I will go find the past article and send if you want.
You couldn't even be bothered to look it up yourself. Makes you even more unreliable.
The SARS-CoV nsp12 Polymerase Active Site Is Tuned for Large-Genome Replication
https://pubmed.ncbi.nlm.nih.gov/35924919/
EDIT: THIS FUCKING IDIOT DOESNT EVEN KNOW WHAT AN ABSTRACT IS IN A RESEARCH PAPER 🤣 WHO GAVE THIS GUY A PEPE?
Is that the ENTIRE paper?
A single paragraph?
Talk about unreliable ...
You don't even know what a paper is supposed to look like.
No "Materials and Methods" section?
Of course not, because it is not a paper. But you don't understand that, do you?
The first sentence:
That is a supposed statement of fact. Notice, it has NO REFERENCE to back it up.
The same is true of every sentence in the paragraph. No references whatsoever. No research to back it up. Just baseless statements of opinion, not science.
This is not a research paper. It is propaganda.
And you don't know how to tell the difference, which is why you are gullible in believing someone's assertions with zero proof.
Holy shit idiot do you know what an abstract is? Learn about how published research papers are written you fucking moron.
It literally says full text link in the link I already gave. An abstract is just a summary of their findings. Its the appetizer thats tasted if someone wants to read further.
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/35924919/
OK ... let's see how much you understand.
Go to "Materials and Methods."
Do you know what that means? Explain.
Do you know what that means? Explain.
Why the proteins, and NOT the "virus?"
Do you know where those proteins came from? Do you know what was MIXED with what, and which proteins were being observed?
Right off the bat, we know they were NOT looking at a "virus," but rather at proteins.
So ... WHICH proteins would be an important thing to know.
Making proteins, not studying a virus.
They are supposedly (according to you) studying RNA virus, using PCR, and yet they are actually looking at proteins -- that were made from a MIXTURE of material, none of which has anything at all to do with a "virus."
RNA is in a lot of things. In this case, bacteria.
See the problem?
It is all ASSUMED to be somehow related to RNA virus -- and that ain't science.
Mixing with E. Coli is not studying a virus.
It is studying a mixture of genetic material.
A MIXTURE.
So ...
They are taking proteins from E. Coli -- NOT FROM SARS-CoV-2 VIRUS.
E Coli is bacteria, not virus.
They claim they can CREATE something "SIMILAR TO" SARS-CoV-2 from this method.
Notice ... they are NOT STARTING WITH A SARS VIRUS.
Why jump through all these hoops? Why not just get a sample of SARS-CoV-2 virus and go from there?
Oh ... because they CAN'T. More than 100 agencies and laboratories around the world ALL have stated on the record that ... NO SUCH SAMPLE EXISTS, ANYWHERE IN THE WORLD.
THAT ... is why they jump through these ridiculous hoops.
Then, they break what they have apart by ADDING CHEMICALS.
Then, they centrifuge and claim they have something worth looking at.
Finally, they draw conclusions about "RNA Virus" based on this MIXTURE of materials that includes CHEMICALS that would not exist inside the body ... if the virus existed.
And they do it all with bacteria, no virus even included.
You are claiming this has some sort of great "proof" related to genomic sequencing and SARS and Covid (ultimately).
In reality, it has nothing to do with the science of so-called virus.
Proteins from bacteria and other materials. So what?
You call it an "awesome paper."
It is garbage.
Please tell me where I called it an "awesome paper". I am just sharing what I find from my sources and on this particular one I get a shitty trash replying back after I explicitly said in the first comment "I am not an expert".
Dont know what it means and never professed I did.
Codon - A sequence of three adjacent nucleotides constituting the genetic code that determines the insertion of a specific amino acid in a polypeptide chain during protein synthesis or the signal to stop protein synthesis.
Yes they are playing around with the amino acid proteins in here.
So they are genetically modifying proteins in E Coli to mimic those of SARS-Cov-2 to understand their mechanism. This very common and much easier way to experiment than with an actual virus that includes all the extra biosafety regulations and costs. In the end you still just want to understand the pieces that make the virus work.
The virus is not a flu, the genes have been sequenced and it was even shown that Modern a had patented sequences of its genes back in 2017. These patented genes do not show up naturally in any form of life prior to the pandemic. This is interesting if you take into account of Monsanto GMO patent court cases in the past, it means Moderna owns SARS-Cov-2 and all the damage that comes with it. You can prove this for yourself with BLAST, I will go find the past article and send if you want.