A T7-based pET-26 plasmid with a N-terminal His8 tag followed by a TEV cleavage site was used
Do you know what that means? Explain.
to express codon optimized versions
Do you know what that means? Explain.
of the Wuhan-1 strain SARS-CoV-2 proteins
Why the proteins, and NOT the "virus?"
Do you know where those proteins came from? Do you know what was MIXED with what, and which proteins were being observed?
Right off the bat, we know they were NOT looking at a "virus," but rather at proteins.
So ... WHICH proteins would be an important thing to know.
The nsp8L7 fusion proteins were made by adding ...
Making proteins, not studying a virus.
They are supposedly (according to you) studying RNA virus, using PCR, and yet they are actually looking at proteins -- that were made from a MIXTURE of material, none of which has anything at all to do with a "virus."
RNA is in a lot of things. In this case, bacteria.
See the problem?
It is all ASSUMED to be somehow related to RNA virus -- and that ain't science.
All proteins were expressed in E. coli BL21(DE3) CodonPlus cells (Agilent Technologies) and grown
Mixing with E. Coli is not studying a virus.
It is studying a mixture of genetic material.
A MIXTURE.
Harvested cells were lysed [broken apart] ... using a ... microfluidizer [machine] ... in a buffer containing [chemicals] ...
So ...
They are taking proteins from E. Coli -- NOT FROM SARS-CoV-2 VIRUS.
E Coli is bacteria, not virus.
They claim they can CREATE something "SIMILAR TO" SARS-CoV-2 from this method.
Notice ... they are NOT STARTING WITH A SARS VIRUS.
Why jump through all these hoops? Why not just get a sample of SARS-CoV-2 virus and go from there?
Oh ... because they CAN'T. More than 100 agencies and laboratories around the world ALL have stated on the record that ... NO SUCH SAMPLE EXISTS, ANYWHERE IN THE WORLD.
THAT ... is why they jump through these ridiculous hoops.
Then, they break what they have apart by ADDING CHEMICALS.
Then, they centrifuge and claim they have something worth looking at.
Finally, they draw conclusions about "RNA Virus" based on this MIXTURE of materials that includes CHEMICALS that would not exist inside the body ... if the virus existed.
And they do it all with bacteria, no virus even included.
You are claiming this has some sort of great "proof" related to genomic sequencing and SARS and Covid (ultimately).
In reality, it has nothing to do with the science of so-called virus.
Proteins from bacteria and other materials. So what?
Please tell me where I called it an "awesome paper". I am just sharing what I find from my sources and on this particular one I get a shitty trash replying back after I explicitly said in the first comment "I am not an expert".
A T7-based pET-26 plasmid with a N-terminal His8 tag followed by a TEV cleavage site was used
Dont know what it means and never professed I did.
to express codon optimized versions
Codon - A sequence of three adjacent nucleotides constituting the genetic code that determines the insertion of a specific amino acid in a polypeptide chain during protein synthesis or the signal to stop protein synthesis.
Yes they are playing around with the amino acid proteins in here.
Coronaviruses (CoV) form large multicomponent RNA replication-transcription complexes containing a core RNA synthesis machine made of the nsp12 RdRP protein with one nsp7 and two nsp8 proteins as essential subunits required for activity. We show that assembly of this complex can be accelerated 5-fold by preincubation of nsp12 with nsp8 and further optimized with the use of a novel nsp8L7 heterodimer fusion protein construct.
All proteins used in this study were expressed in Escherichia coli using T7 promoters and purified through metal-affinity, ion-exchange, and size-exclusion chromatography (Fig. 2A).
So they are genetically modifying proteins in E Coli to mimic those of SARS-Cov-2 to understand their mechanism. This very common and much easier way to experiment than with an actual virus that includes all the extra biosafety regulations and costs. In the end you still just want to understand the pieces that make the virus work.
The virus is not a flu, the genes have been sequenced and it was even shown that Modern a had patented sequences of its genes back in 2017. These patented genes do not show up naturally in any form of life prior to the pandemic. This is interesting if you take into account of Monsanto GMO patent court cases in the past, it means Moderna owns SARS-Cov-2 and all the damage that comes with it. You can prove this for yourself with BLAST, I will go find the past article and send if you want.
Please tell me where I called it an "awesome paper".
Read the title of YOUR thread.
Dont know what it means and never professed I did.
Yeah, that's what I thought.
So they are genetically modifying proteins in E Coli to mimic those of SARS-Cov-2
And THAT is a BIG problem.
E Coli is bacteria, nothing to do with virus.
Why not just start with the DAMN VIRUS?
Because they can't.
Why can't they?
Because a sample of the virus ... DOES NOT EXIST IN ANY LAB ANYWHERE IN THE WORLD.
That's a bit of a problem ...
In the end you still just want to understand the pieces that make the virus work.
Wrong.
FIRST, it must be established that a virus ... EXISTS.
NEXT, we take it apart to look at ITS proteins -- not the proteins of something ELSE.
THEN ... MAYBE we can arrive at some conclusions.
Not before.
The virus is not a flu, the genes have been sequenced
False. The "genetic sequence" was generated via computer program, NOT from a real, physical thing in the real, physical world. ALL done inside a virtual computer software program. ALL of it.
I could also generate a genomic sequence of a unicorn. Would you insist that I FIRST show you a real unicorn, take genetic material from that unicorn, and do a genetic sequence from THAT real, physical thing?
You SHOULD.
If not, then you are not following science, only dogma.
Virologists have NEVER taken a genetic sequence from a real, physical "virus."
Always done via computer software program ... ONLY.
Modern a had patented sequences ...
Again, ALL MADE UP BY A COMPUTER PROGRAM.
They NEVER had a real sample of it -- because there isn't one!
... back in 2017
That's because the SARS 2 was made from SARS 1 (2003), BOTH from a computer program and NOT from a real, physical thing existing in the real, three dimensional world.
THIS IS WHY THEY MUST USE THESE "SURROGATE" EXPERIMENTS, and NOT use the "real virus" -- which DOES NOT EXIST.
Moderna owns SARS-Cov-2 and all the damage that comes with it.
There is no damage from something that does not exist.
But Moderna DOES own the vaccines, which are harmful drugs, and the executives and "scientists" at Moderna should be prosecuted for their crimes.
You can prove this for yourself with BLAST, I will go find the past article and send if you want.
No, because you continue to IGNORE the evidence that ALL of this is made up nonsense.
I could send you pictures of unicorns, if you want.
This is a quote from the Twitter post. Not my words. I'm doing my rounds of posting any research posted by my sources. I'm not hand making every single title, I'm providing the information here so other people can see it if they want to.
Yeah, that's what I thought.
I literally said that in my first comment on this thread that I'm no expert you fucking trash.
BECAUSE ITS FUCKING EXPENSIVE AND REQUIRES A LEVEL 4 LAB WHEN ALL THEY ARE DOING IS FIGURING OUT THE MECHANISM OF PROTEINS AND GENE SEQUENCES THAT MAKE UP THE VIRUS. THE MECHANISM WORKS THE SAME E COLI OR ANY OTHER CELL BUT E COLI IS CHEAP AND EASY TO WORK WITH.
False. The "genetic sequence" was generated via computer program, NOT from a real, physical thing in the real, physical world. ALL done inside a virtual computer software program. ALL of it.
I could also generate a genomic sequence of a unicorn. Would you insist that I FIRST show you a real unicorn, take genetic material from that unicorn, and do a genetic sequence from THAT real, physical thing?
Covid Origins Smoking Gun
How to BLAST your way to the truth about the origins of COVID-19
BLAST Search Results.Replicate and find the CTCCTCGGCGGGCACGTAG Moderna synthetic genome patents and link to Covid. WO2020 and WO2021 and their patents are related to after the Sars-Cov-2 outbreak (2020 and thereafter) and can be dismissed. Interestingly, this sequence doesn't show up in natural life at all before Covid-19, only shows in Moderna patents, and the gene is involved in the virus invading the human cell
@_Bilbobagga
Previous court case has ruled if your patent is found in something you own it. With a 2017 Moderna patent found in COVID-19 genome, Moderna can be found responsible for COVID-19! Lucky they have made billions off the vaccine for the virus they created, might need it and more!
No flu permanently takes away your sense of smell or taste. Covid-19 does in rare cases. I'm not aware of any flu that that takes away your sense 6 months after symptoms first occur. It's a fucking real virus and it was genetically engineered but stupidly. The Furin Cleavage Site (FCS) is the key to this, the FCS did not occur naturally and is what allows the virus to enter human cells.
There are level 4 labs that do handle SARS-Cov-2 and Coronaviruses. Again VERY FUCKING EXPENSIVE to handle for understanding every protein and gene sequence so it's not done as often.
There is a real virus people get sick off of. Other people around them get sick after they get sick. Take Ivermectin (which is anti-viral) and everything is fine. Go to Conspiracies.win if you think there is no SARS-Cov-2 virus, it has been sequenced by multiple 3rd parties since the initial Illumina 4chan threads were made
Fauci docs have been leaked with proof that they performed gain of function research on coronaviruses that we precursors to SARS-Cov-2
Have you been paying any fucking attention at all??
A link to Clandestine's twitter thread about the emails released via FOIA pertaining to Covid-19 showing the connection with Fauci/ NIH & Wuhan
I’m not sure what is going on but in the past 4 months, Russia and China accused the US of creating bioweapons at their borders and none of us heard about and our media wrote it off as “disinformation” and largely didn’t report on it other than to call it a conspiracy theory.
For all of this to make sense, you must be privy to the reality that the US NIH did indeed create C19 in Wuhan. Which has been largely accepted as the reality and confirmed in the emails between Collins and Fauci.
Thanks to @Jim_Jordan and @JamesComer , we have the emails sent to Fauci and Collins pertaining to Covid 19 origins.
Ones I Find particularly interesting
Dr. Mike Farzan, Discoverer of SARS receptor, Harvard Medical, Immunology and Microbiology at University of Florida.
His initial assessment of C-19:
-furin site is unnatural and likely man made
-furin site manipulation enabled human adaption and transmission
Dr. Andrew Rambaut, Evolutionary Biologist and Professor of Molecular Evolution at University if Edinburg.
-“unusual furin cleavage site” suggesting C19 was created
-can’t confirm natural occurrence without evidence
-should defer to experts working with the virus in Wuhan
@KathMLee1
There's the issue of the HIV-1 GP120 envelope and GAG inserts in the spike protein of the virus. The author was bullied into retracting his paper. It needs to be resubmitted. I've posted about acute hiv infection mimicking vaccine side effects, false positive HIV, etc
BECAUSE ITS FUCKING EXPENSIVE AND REQUIRES A LEVEL 4 LAB WHEN ALL THEY ARE DOING IS FIGURING OUT THE MECHANISM OF PROTEINS AND GENE SEQUENCES THAT MAKE UP THE VIRUS.
Nonsense. It's a lie to hide the fact that ... THERE IS NO VIRUS AVAILABLE TO STUDY.
Period.
THE MECHANISM WORKS THE SAME E COLI OR ANY OTHER CELL BUT E COLI IS CHEAP AND EASY TO WORK WITH.
No proof that that is true. It is pure SPECULATION.
If you (a) do NOT have a physical virus sample, then you (b) do NOT know what proteins it might have, and therefore, (c) cannot ASSUME that a potato or unicorn protein would be the same as a theoretical virus protein -- much less, a bacteria protein.
Why not just use the proteins from fairy dust, and call it a day?
It is all ... MADE UP.
BLAST Search Results.Replicate and find the CTCCTCGGCGGGCACGTAG Moderna synthetic genome patents and link to Covid.
Jesus Christ! Why do you not understand something so basic?
"BLAST" is an acronym for: Basic Local Alignment Search Tool. It is nothing more than a database.
That database has FAKE genomes in it -- created from nothing but a computer program.
Why don't you look up the BLAST "proof" of the unicorn genome?
If a database has FAKE data in it, then guess what you will find when you search it? You will find FAKE data.
Interestingly, this sequence doesn't show up in natural life at all before Covid-19, only shows in Moderna patents
Right! Because it is FUCKING CREATED BY A COMPUTER PROGRAM.
It does NOT EXIST in the physical world of reality.
No flu permanently takes away your sense of smell or taste.
False. Temporary loss of smell and taste has been reported long before Covid. Not common, but reported for many years before.
Thought by some to be linked to nerve damage, which is linked to taste and smell.
It's a fucking real virus and it was genetically engineered but stupidly.
That's an opinion, with zero research to back it up.
There is a real virus people get sick off of.
Again, another opinion with zero research to back it up.
Just an opinion.
if you think there is no SARS-Cov-2 virus, it has been sequenced by multiple 3rd parties
False. There has NEVER been a genomic sequence of a PHYSICAL VIRUS.
ALL of the sequences of the variations and "original" have been CREATED IN A COMPUTER SOFTWARE PROGRAM -- with NO original virus.
If you STILL do not understand this basic issue, then there is no point in our further discussion.
I challenged your knowledge mostly so other people reading this might benefit. We all need to learn how to unravel the lies that are everywhere around us, with "mainstream" virology being one of the chief culprits.
OK ... let's see how much you understand.
Go to "Materials and Methods."
Do you know what that means? Explain.
Do you know what that means? Explain.
Why the proteins, and NOT the "virus?"
Do you know where those proteins came from? Do you know what was MIXED with what, and which proteins were being observed?
Right off the bat, we know they were NOT looking at a "virus," but rather at proteins.
So ... WHICH proteins would be an important thing to know.
Making proteins, not studying a virus.
They are supposedly (according to you) studying RNA virus, using PCR, and yet they are actually looking at proteins -- that were made from a MIXTURE of material, none of which has anything at all to do with a "virus."
RNA is in a lot of things. In this case, bacteria.
See the problem?
It is all ASSUMED to be somehow related to RNA virus -- and that ain't science.
Mixing with E. Coli is not studying a virus.
It is studying a mixture of genetic material.
A MIXTURE.
So ...
They are taking proteins from E. Coli -- NOT FROM SARS-CoV-2 VIRUS.
E Coli is bacteria, not virus.
They claim they can CREATE something "SIMILAR TO" SARS-CoV-2 from this method.
Notice ... they are NOT STARTING WITH A SARS VIRUS.
Why jump through all these hoops? Why not just get a sample of SARS-CoV-2 virus and go from there?
Oh ... because they CAN'T. More than 100 agencies and laboratories around the world ALL have stated on the record that ... NO SUCH SAMPLE EXISTS, ANYWHERE IN THE WORLD.
THAT ... is why they jump through these ridiculous hoops.
Then, they break what they have apart by ADDING CHEMICALS.
Then, they centrifuge and claim they have something worth looking at.
Finally, they draw conclusions about "RNA Virus" based on this MIXTURE of materials that includes CHEMICALS that would not exist inside the body ... if the virus existed.
And they do it all with bacteria, no virus even included.
You are claiming this has some sort of great "proof" related to genomic sequencing and SARS and Covid (ultimately).
In reality, it has nothing to do with the science of so-called virus.
Proteins from bacteria and other materials. So what?
You call it an "awesome paper."
It is garbage.
Please tell me where I called it an "awesome paper". I am just sharing what I find from my sources and on this particular one I get a shitty trash replying back after I explicitly said in the first comment "I am not an expert".
Dont know what it means and never professed I did.
Codon - A sequence of three adjacent nucleotides constituting the genetic code that determines the insertion of a specific amino acid in a polypeptide chain during protein synthesis or the signal to stop protein synthesis.
Yes they are playing around with the amino acid proteins in here.
So they are genetically modifying proteins in E Coli to mimic those of SARS-Cov-2 to understand their mechanism. This very common and much easier way to experiment than with an actual virus that includes all the extra biosafety regulations and costs. In the end you still just want to understand the pieces that make the virus work.
The virus is not a flu, the genes have been sequenced and it was even shown that Modern a had patented sequences of its genes back in 2017. These patented genes do not show up naturally in any form of life prior to the pandemic. This is interesting if you take into account of Monsanto GMO patent court cases in the past, it means Moderna owns SARS-Cov-2 and all the damage that comes with it. You can prove this for yourself with BLAST, I will go find the past article and send if you want.
Read the title of YOUR thread.
Yeah, that's what I thought.
And THAT is a BIG problem.
E Coli is bacteria, nothing to do with virus.
Why not just start with the DAMN VIRUS?
Because they can't.
Why can't they?
Because a sample of the virus ... DOES NOT EXIST IN ANY LAB ANYWHERE IN THE WORLD.
That's a bit of a problem ...
Wrong.
FIRST, it must be established that a virus ... EXISTS.
NEXT, we take it apart to look at ITS proteins -- not the proteins of something ELSE.
THEN ... MAYBE we can arrive at some conclusions.
Not before.
False. The "genetic sequence" was generated via computer program, NOT from a real, physical thing in the real, physical world. ALL done inside a virtual computer software program. ALL of it.
I could also generate a genomic sequence of a unicorn. Would you insist that I FIRST show you a real unicorn, take genetic material from that unicorn, and do a genetic sequence from THAT real, physical thing?
You SHOULD.
If not, then you are not following science, only dogma.
Virologists have NEVER taken a genetic sequence from a real, physical "virus."
Always done via computer software program ... ONLY.
Again, ALL MADE UP BY A COMPUTER PROGRAM.
They NEVER had a real sample of it -- because there isn't one!
That's because the SARS 2 was made from SARS 1 (2003), BOTH from a computer program and NOT from a real, physical thing existing in the real, three dimensional world.
THIS IS WHY THEY MUST USE THESE "SURROGATE" EXPERIMENTS, and NOT use the "real virus" -- which DOES NOT EXIST.
There is no damage from something that does not exist.
But Moderna DOES own the vaccines, which are harmful drugs, and the executives and "scientists" at Moderna should be prosecuted for their crimes.
No, because you continue to IGNORE the evidence that ALL of this is made up nonsense.
I could send you pictures of unicorns, if you want.
This is a quote from the Twitter post. Not my words. I'm doing my rounds of posting any research posted by my sources. I'm not hand making every single title, I'm providing the information here so other people can see it if they want to.
I literally said that in my first comment on this thread that I'm no expert you fucking trash.
https://greatawakening.win/p/15JTyU37T9/x/c/4OdgvYnlGxI
BECAUSE ITS FUCKING EXPENSIVE AND REQUIRES A LEVEL 4 LAB WHEN ALL THEY ARE DOING IS FIGURING OUT THE MECHANISM OF PROTEINS AND GENE SEQUENCES THAT MAKE UP THE VIRUS. THE MECHANISM WORKS THE SAME E COLI OR ANY OTHER CELL BUT E COLI IS CHEAP AND EASY TO WORK WITH.
Covid Origins Smoking Gun
How to BLAST your way to the truth about the origins of COVID-19
https://arkmedic.substack.com/p/how-to-blast-your-way-to-the-truth
https://archive.fo/xj5xk (edited)
BLAST Search Results.Replicate and find the CTCCTCGGCGGGCACGTAG Moderna synthetic genome patents and link to Covid. WO2020 and WO2021 and their patents are related to after the Sars-Cov-2 outbreak (2020 and thereafter) and can be dismissed. Interestingly, this sequence doesn't show up in natural life at all before Covid-19, only shows in Moderna patents, and the gene is involved in the virus invading the human cell
https://blast.ncbi.nlm.nih.gov/Blast.cgi
@JikkyKjj Who owns #SARSCoV2?
If there is a contaminant that is patented, the patent owner owns it.
If you own something that harms someone else, what happens?
Monsanto may have dropped #BigPharma into the biggest pile of BatPile of pooin criminal history. #CTCCTCGGCGGGCACGTAG
https://twitter.com/the_coopertron/status/1482702820293988353
https://archive.ph/D5Q64
@_Bilbobagga Previous court case has ruled if your patent is found in something you own it. With a 2017 Moderna patent found in COVID-19 genome, Moderna can be found responsible for COVID-19! Lucky they have made billions off the vaccine for the virus they created, might need it and more!
https://twitter.com/_Bilbobagga/status/1482662636005519360?s=20
https://archive.ph/q0u1n
No flu permanently takes away your sense of smell or taste. Covid-19 does in rare cases. I'm not aware of any flu that that takes away your sense 6 months after symptoms first occur. It's a fucking real virus and it was genetically engineered but stupidly. The Furin Cleavage Site (FCS) is the key to this, the FCS did not occur naturally and is what allows the virus to enter human cells.
There are level 4 labs that do handle SARS-Cov-2 and Coronaviruses. Again VERY FUCKING EXPENSIVE to handle for understanding every protein and gene sequence so it's not done as often.
There is a real virus people get sick off of. Other people around them get sick after they get sick. Take Ivermectin (which is anti-viral) and everything is fine. Go to Conspiracies.win if you think there is no SARS-Cov-2 virus, it has been sequenced by multiple 3rd parties since the initial Illumina 4chan threads were made
https://archive.ph/wefGD
https://archive.ph/NfFW2
https://archive.is/O2oxl
https://archive.ph/93JkP
Fauci docs have been leaked with proof that they performed gain of function research on coronaviruses that we precursors to SARS-Cov-2
Have you been paying any fucking attention at all??
A link to Clandestine's twitter thread about the emails released via FOIA pertaining to Covid-19 showing the connection with Fauci/ NIH & Wuhan
I broke down their official NIH emails released by Congress https://archive.ph/kyR2q#selection-1575.1-1579.64
which leads to
https://archive.ph/x7Gp2
https://republicans-oversight.house.gov/wp-content/uploads/2022/01/Letter-Re.-Feb-1-Emails-011122.pdf
https://web.archive.org/web/20220118110039/https://republicans-oversight.house.gov/wp-content/uploads/2022/01/Letter-Re.-Feb-1-Emails-011122.pdf
Thanks to @Jim_Jordan and @JamesComer , we have the emails sent to Fauci and Collins pertaining to Covid 19 origins.
Ones I Find particularly interesting
His initial assessment of C-19: -furin site is unnatural and likely man made -furin site manipulation enabled human adaption and transmission
-“unusual furin cleavage site” suggesting C19 was created -can’t confirm natural occurrence without evidence -should defer to experts working with the virus in Wuhan
@KathMLee1
There's the issue of the HIV-1 GP120 envelope and GAG inserts in the spike protein of the virus. The author was bullied into retracting his paper. It needs to be resubmitted. I've posted about acute hiv infection mimicking vaccine side effects, false positive HIV, etc
https://pbs.twimg.com/media/FLMRW80XoAU16Vb?format=jpg&name=medium
https://twitter.com/KathMLee1/status/1491555383613308933
https://archive.ph/kugC7
@JikkyKjj The epitopes on the binding sites of the virus are from HIV-1. So antibodies to #SARSCOV2 are likely to cross react with HIV antibodies.
This is not random.
https://twitter.com/JikkyKjj/status/1491730672335003648
https://web.archive.org/web/20220210111123/https://twitter.com/JikkyKjj/status/1491730672335003648
Nonsense. It's a lie to hide the fact that ... THERE IS NO VIRUS AVAILABLE TO STUDY.
Period.
No proof that that is true. It is pure SPECULATION.
If you (a) do NOT have a physical virus sample, then you (b) do NOT know what proteins it might have, and therefore, (c) cannot ASSUME that a potato or unicorn protein would be the same as a theoretical virus protein -- much less, a bacteria protein.
Why not just use the proteins from fairy dust, and call it a day?
It is all ... MADE UP.
Jesus Christ! Why do you not understand something so basic?
"BLAST" is an acronym for: Basic Local Alignment Search Tool. It is nothing more than a database.
That database has FAKE genomes in it -- created from nothing but a computer program.
Why don't you look up the BLAST "proof" of the unicorn genome?
If a database has FAKE data in it, then guess what you will find when you search it? You will find FAKE data.
Right! Because it is FUCKING CREATED BY A COMPUTER PROGRAM.
It does NOT EXIST in the physical world of reality.
False. Temporary loss of smell and taste has been reported long before Covid. Not common, but reported for many years before.
Thought by some to be linked to nerve damage, which is linked to taste and smell.
That's an opinion, with zero research to back it up.
Again, another opinion with zero research to back it up.
Just an opinion.
False. There has NEVER been a genomic sequence of a PHYSICAL VIRUS.
ALL of the sequences of the variations and "original" have been CREATED IN A COMPUTER SOFTWARE PROGRAM -- with NO original virus.
If you STILL do not understand this basic issue, then there is no point in our further discussion.
I challenged your knowledge mostly so other people reading this might benefit. We all need to learn how to unravel the lies that are everywhere around us, with "mainstream" virology being one of the chief culprits.