I think you are completely misunderstanding how viruses are grown and isolated in a lab. Each type of virus has one or more varieties of cells that it is best able to infect and replicate in. For example, influenza viruses do best in canine kidney cells, so researchers grow canine kidney cells in flasks and then infect those cells with influenza virus to grow large quantities of virus particles to be used in research. Many other types of viruses grow best in kidney cells isolated from the African green monkey, and some grow well in specific cancer cells. Once you have large stocks of virus grown, those can be used for many different types of research, including isolating the virus particles themselves to study their structure and life cycle. Our labs are strictly used for testing experimental drugs against many dozens of different viruses. We don't develop or create the drugs, we are just paid to test them against specific viruses. We don't manipulate the viruses, we just grow stocks of them so that we can then use those stocks to test the experimental drugs.
We have 3 entire labs dedicated strictly to SARS-CoV-2 (COVID-19) testing. Of course the virus has been isolated. If the virus hadn't been isolated, then no testing would be possible. Isolated samples of many different viruses are available to approved research facilities from ATCC (American Type Culture Collection) and several other sources. The more dangerous viruses require government permission to obtain and work with and labs using them are strictly regulated and frequently inspected for safety and security by the federal government.
People who claim that viruses, including COVID, have not been isolated are completely wrong, and in most cases are probably the same people who believe the earth is flat and that men can get pregnant. Individual virus particles can be viewed using electron microscopy, and the entire genome of a virus can easily be mapped and studied. It's ridiculous to claim that viruses haven't been isolated.
I found a study earlier today (while on my comp) that suggested arganine inhibits sars 2.0 (I'm on mobile and lazy, so yeah, lol).
From other info I've come across, arganine actually increases replication of herpes viruses, which are one of the possible causes of chronic fatigue/fibromyalgia. Any indication of the effect of lysine, which supposedly inhibits herpes viruses from replicating, on sars 2.0?
Seems like they would be a pretty scary thing for cfs/fibro patients if lysine makes the coof replicate faster...
Also, sorry for the off the beaten path question, lol.
I read a very good paper last year about the effects of arginine and lysine on viral activity. Again, I don't have a link to the paper handy, but I included a link to some abstracts and some other publications below. Arginine seems to increase the rate at which virus particles can infect cells, making it harder to clear an infection. Lysine does the opposite, it seems to decrease the infection rate, thus making it easier for your immune system + any medications to clear an infection. I personally take a low dose of 500 mg of L-lysine daily. One paper I read claimed that doses of up to 3,000-4,000 mg daily of L-lysine is safe, and the upper safe limit could be even higher. I'm not a doctor, but I know that I personally have never had any adverse effects at 500 mg daily.
Both Arginine and Lysine are found in moderate to high levels in many foods, and so you may want to limit your intake of foods with arginine, and increase your intake of foods with lysine. Most of the foods I commonly eat are high in lysine (meats and cheeses to name a couple), and I generally don't eat many foods that contain a lot of arginine, so I didn't have to change anything about my diet. I have chosen to supplement L-lysine and do nothing to raise or lower my L-arginine levels, and so far that seems to be working for me. But remember, I'm not a doctor, this is just from my personal experience.
There are some studies that suggest that arginine can actually be helpful against SARS-CoV-2 by improving endothelial function, so the evidence is conflicting. You'll have to do your own research and decide for yourself. I included links to some abstracts and some other links I have handy that you might be interested in. Many of these links have been posted on GAW before, so you can find a lot of good information by searching here.
Ok, so I think you misunderstand me. I am not on any particular bandwagon. And before you compare me again to a flat earther, which is just ad hominem, I asked you what do you think about mixing a sample of a person's lung lavage with animal cells or sick human cells, antibiotics etc. in order to 'isolate it'. you must admit this is counterintuitive, and there are methodologies that do not do this. Also, I have seen methodologies that add a wide mixture of these foreign cells.
You claim that in your lab, only one type of cell is grown for each specific virus (well of course the 'host cells are replicated for this purpose, this is why the human cancer cells have lasted all these years, and why that particular cancer was chosen, because it is particularly virulent and therefore replicating rapidly). So that's interesting. This is why I asked you to elaborate, because it is hard to find this type of information.
As for my request for sauce - as you admitted previously, there is a lot of information crowding into this space, and it is confusing, even for someone trained to read methodologies and critique them. So, I again re-iterate what are your sources apart from, but as well as your personal experience? So, I am hoping that you can get to a computer and reveal to us the literature you are using.
I wasn't comparing you to a flat earther, there was no ad hominem attack. I took your comment to mean that you hadn't made up your mind what to believe and were asking my opinion, and I was simply pointing out that the many people I see post on this site (not you) that insist that no virus has ever been isolated are ridiculously misinformed and have the tendency to believe every wild ass thing they see on a youtube video and it's impossible to change their mind. The point of my comment is that you should not believe these people, they seem to read something on the internet and just believe it without any knowledge or understanding of whether it comes from an intelligent source or a complete moron.
In our labs, we focus on high-throughput screening so that we can test thousands of experimental compounds per year in vitro (cell culture). Less than one percent of those compounds are selected to go into a mouse infection model for further study. We don't do much isolation of viruses, we obtain virus isolates from other sources for our work, and so I am not familiar with the current methods being used for such work, but clearly we couldn't get all our virus isolates if they didn't exist. I can't comment on your question about some of the procedures you've read about on that topic.
For each virus, we use the cell line that the virus grows best in for us to perform our testing. High throughput screening, we just want to know if a compound shows any activity against the virus or not. We're generally not looking for more detail than that, so I can't answer all of your questions. Seed the cells on a tissue culture plate, and 24 hours later add the drugs to the cells and then infect the cells with the virus and observe what happens.
My source is simply 30 years of working in virology labs doing the sort of testing I've described. I'm sorry if that's not the type of source you're looking for. I read papers online, especially since all the COVID bullshit started, and those are available to anyone who wants to search for them. I don't keep a database of everything I read, and I gave you the few links that I had bookmarked and commented on some of the others from memory (which is not always perfect).
I think you are completely misunderstanding how viruses are grown and isolated in a lab. Each type of virus has one or more varieties of cells that it is best able to infect and replicate in. For example, influenza viruses do best in canine kidney cells, so researchers grow canine kidney cells in flasks and then infect those cells with influenza virus to grow large quantities of virus particles to be used in research. Many other types of viruses grow best in kidney cells isolated from the African green monkey, and some grow well in specific cancer cells. Once you have large stocks of virus grown, those can be used for many different types of research, including isolating the virus particles themselves to study their structure and life cycle. Our labs are strictly used for testing experimental drugs against many dozens of different viruses. We don't develop or create the drugs, we are just paid to test them against specific viruses. We don't manipulate the viruses, we just grow stocks of them so that we can then use those stocks to test the experimental drugs.
We have 3 entire labs dedicated strictly to SARS-CoV-2 (COVID-19) testing. Of course the virus has been isolated. If the virus hadn't been isolated, then no testing would be possible. Isolated samples of many different viruses are available to approved research facilities from ATCC (American Type Culture Collection) and several other sources. The more dangerous viruses require government permission to obtain and work with and labs using them are strictly regulated and frequently inspected for safety and security by the federal government.
People who claim that viruses, including COVID, have not been isolated are completely wrong, and in most cases are probably the same people who believe the earth is flat and that men can get pregnant. Individual virus particles can be viewed using electron microscopy, and the entire genome of a virus can easily be mapped and studied. It's ridiculous to claim that viruses haven't been isolated.
I found a study earlier today (while on my comp) that suggested arganine inhibits sars 2.0 (I'm on mobile and lazy, so yeah, lol).
From other info I've come across, arganine actually increases replication of herpes viruses, which are one of the possible causes of chronic fatigue/fibromyalgia. Any indication of the effect of lysine, which supposedly inhibits herpes viruses from replicating, on sars 2.0?
Seems like they would be a pretty scary thing for cfs/fibro patients if lysine makes the coof replicate faster...
Also, sorry for the off the beaten path question, lol.
I read a very good paper last year about the effects of arginine and lysine on viral activity. Again, I don't have a link to the paper handy, but I included a link to some abstracts and some other publications below. Arginine seems to increase the rate at which virus particles can infect cells, making it harder to clear an infection. Lysine does the opposite, it seems to decrease the infection rate, thus making it easier for your immune system + any medications to clear an infection. I personally take a low dose of 500 mg of L-lysine daily. One paper I read claimed that doses of up to 3,000-4,000 mg daily of L-lysine is safe, and the upper safe limit could be even higher. I'm not a doctor, but I know that I personally have never had any adverse effects at 500 mg daily.
Both Arginine and Lysine are found in moderate to high levels in many foods, and so you may want to limit your intake of foods with arginine, and increase your intake of foods with lysine. Most of the foods I commonly eat are high in lysine (meats and cheeses to name a couple), and I generally don't eat many foods that contain a lot of arginine, so I didn't have to change anything about my diet. I have chosen to supplement L-lysine and do nothing to raise or lower my L-arginine levels, and so far that seems to be working for me. But remember, I'm not a doctor, this is just from my personal experience.
There are some studies that suggest that arginine can actually be helpful against SARS-CoV-2 by improving endothelial function, so the evidence is conflicting. You'll have to do your own research and decide for yourself. I included links to some abstracts and some other links I have handy that you might be interested in. Many of these links have been posted on GAW before, so you can find a lot of good information by searching here.
Arginine and Lysine vs SARS-CoV-2
https://pubmed.ncbi.nlm.nih.gov/34372507/
https://pubmed.ncbi.nlm.nih.gov/34836206/
https://pubmed.ncbi.nlm.nih.gov/33160064/
https://pubmed.ncbi.nlm.nih.gov/35868478/
https://www.pnas.org/doi/pdf/10.1073/pnas.2101708118
https://www.thelancet.com/journals/eclinm/article/PIIS2589-5370(21)00405-3/fulltext
Vitamin D vs SARS-CoV-2
https://www.sciencedirect.com/science/article/pii/S0188440922000455
Vitamin C
https://www.bitchute.com/video/W1TJl4syE7wx/
SARS-CoV-2 vaccines and IgG antibodies
https://igorchudov.substack.com/p/booster-caused-immune-tolerance-explains
Ivermectin promotes wound healing
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6210064/#:~:text=Ivermectin%20could%20also%20be%20explored,resection%20or%20spinal%20cord%20injury.
All mRNA vaccines cause harm
https://rumble.com/v21ppz6-all-mrna-vaccines-will-cause-harm-prof.-dr.-bhakdi.html
Ivermectin is a cancer wonder drug
https://jeffreydachmd.com/2016/05/ivermectin-antiparasitic-anticancer-wonder-drug/
Ok, so I think you misunderstand me. I am not on any particular bandwagon. And before you compare me again to a flat earther, which is just ad hominem, I asked you what do you think about mixing a sample of a person's lung lavage with animal cells or sick human cells, antibiotics etc. in order to 'isolate it'. you must admit this is counterintuitive, and there are methodologies that do not do this. Also, I have seen methodologies that add a wide mixture of these foreign cells.
You claim that in your lab, only one type of cell is grown for each specific virus (well of course the 'host cells are replicated for this purpose, this is why the human cancer cells have lasted all these years, and why that particular cancer was chosen, because it is particularly virulent and therefore replicating rapidly). So that's interesting. This is why I asked you to elaborate, because it is hard to find this type of information.
As for my request for sauce - as you admitted previously, there is a lot of information crowding into this space, and it is confusing, even for someone trained to read methodologies and critique them. So, I again re-iterate what are your sources apart from, but as well as your personal experience? So, I am hoping that you can get to a computer and reveal to us the literature you are using.
I wasn't comparing you to a flat earther, there was no ad hominem attack. I took your comment to mean that you hadn't made up your mind what to believe and were asking my opinion, and I was simply pointing out that the many people I see post on this site (not you) that insist that no virus has ever been isolated are ridiculously misinformed and have the tendency to believe every wild ass thing they see on a youtube video and it's impossible to change their mind. The point of my comment is that you should not believe these people, they seem to read something on the internet and just believe it without any knowledge or understanding of whether it comes from an intelligent source or a complete moron.
In our labs, we focus on high-throughput screening so that we can test thousands of experimental compounds per year in vitro (cell culture). Less than one percent of those compounds are selected to go into a mouse infection model for further study. We don't do much isolation of viruses, we obtain virus isolates from other sources for our work, and so I am not familiar with the current methods being used for such work, but clearly we couldn't get all our virus isolates if they didn't exist. I can't comment on your question about some of the procedures you've read about on that topic.
For each virus, we use the cell line that the virus grows best in for us to perform our testing. High throughput screening, we just want to know if a compound shows any activity against the virus or not. We're generally not looking for more detail than that, so I can't answer all of your questions. Seed the cells on a tissue culture plate, and 24 hours later add the drugs to the cells and then infect the cells with the virus and observe what happens.
My source is simply 30 years of working in virology labs doing the sort of testing I've described. I'm sorry if that's not the type of source you're looking for. I read papers online, especially since all the COVID bullshit started, and those are available to anyone who wants to search for them. I don't keep a database of everything I read, and I gave you the few links that I had bookmarked and commented on some of the others from memory (which is not always perfect).